DS-Fi3 camera - Compact, high-definition, high-speed and high-sensitivity C-mount camera.

nikon metrology industrial microscopes cameras DS Fi3Nikon's DS-Fi3 is a new high-definition color microscope camera equipped with a 5.9 megapixel CMOS image sensor. Its high-speed data readout, superior color reproduction and high quantum efficiency are optimal for imaging in various observations, such as brightfield, DIC, phase contrast and fluorescence observation.

Benefits and Features

High Definition Imaging

nikon metrology industrial microscopes cameras liquid crystal panel DS Fi3Liquid crystal panel (Objective: TU Plan Fluor 10x)The DS-Fi3 is equipped with a 5.9 megapixel CMOS image sensor, which enables the capture of high-definition images of up to 2880 x 2048 pixels. With a new CMOS image sensor and high-speed data transfer via USB3.0, the DS-Fi3 enables fast focusing even in high-resolution imaging, and efficient image acquisition when using a wide range of illumination techniques.

High Sensitivity and Low Noise

The DS-Fi3 has significantly higher quantum efficiency and lower readout noise than conventional models. It allows the capture of brighter images with higher S/N ratios in fluorescence observation.

nikon metrology industrial microscopes cameras high sensitivity low noise DS Fi3

High-speed Live Display

Due to the high-speed data readout by the CMOS image sensor and high-speed USB3.0 data transfer, the DS-Fi3 displays live images of full 2880 x 2048-pixel images at 15 fps, and 1440 x 1024-pixel images at 30 fps. This enables smooth display during focusing and the selection of observation locations.

nikon metrology industrial microscopes cameras tu plan fluor DS Fi3Mounting plate (Objective: TU Plan Fluor 5x)

Excellent Color Reproducibility

Nikon is well-known for outstanding and lifelike color reproduction, and developing superior algorithms for creating results that look like the actual samples. These algorithms are used in all of the color cameras in the Digital Sight lineup.

nikon metrology industrial microscopes cameras uterine cervix DS Fi3Uterine cervix Pap staining (Objective: CFI Plan Apochromat λ 60x Oil) Photos courtesy of: Kazuhiro Mita, Department of Pathology, Yokohama City University Hospital nikon metrology industrial microscopes cameras bone marrow DS Fi3Bone marrow (Objective: CFI Plan Achromat NCG 40x) Photos courtesy of: Clinical Laboratory Department, Yokohama City University Hospital

Direct connection to a PC

The DS-Fi3 allows direct connection to a PC via a USB3.0 interface to control the camera and capture images using the imaging software NIS-Elements.



Image sensor

1/1.8 inch Color CMOS image sensor
Size: 6.91 x 4.92 mm

Recordable pixels

All pixels: 2880 x 2048
2 vertical and 2 horizontal pixels average: 1440 x 1024

Lens mount


ISO sensitivity
(recommended exposure index)

Standard: equivalent to ISO 50
(Selectable from ISO 50 to ISO 3200 equivalent)

Live display mode*
(Maximum fps)

All pixels (2880 x 2048): 15 fps
 2 vertical and 2 horizontal pixels average: 30 fps
(1440 x 1024): 30 fps

Exposure time

100μ sec to 30 sec

Photometry mode

Average photometry: Average intensity within the photometry area
Peak photometry: Maximum intensity within the photometry area

Exposure control

One-time automatic exposure: Exposure time is adjusted automatically for one-time within the optimum range for the camera
 Continuous automatic exposure: Automatic exposure adjustment is performed continuously to keep the exposure within the camera
Manual exposure: Exposure time and gain settings are made manually

Exposure correction

Average metering: ±1EV, Step: 1/6EV
Peak hold metering: -1EV to ±0EV


USB3.0 (connect with PC, DS-L4) x1, External trigger x1

Power Supply

AC100-240V 50Hz/60Hz

Power Consumption



100 (W) x 66 (D) x 65 (H) mm


400g (approx.)

Operating environment

0-40°C , 60% RH max. (without condensation)

* Maximum frame rate depends on exposure time.



Case studies


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